Preparation, Characterization and Evaluation of Guar Films Impregnated with Relaxing Peptide Loaded into Chitosan Microparticles

Background: Biological molecules such as therapeutic proteins and peptides have provided ample opportunities for further improvements in health wellbeing. However, biomacromolecules face stability challenges regarding premature processing digestion, whenever any enteric delivery route is considered. Hence, oral guar films entrapping peptide-containing chitosan microparticles been developed a new system to carry protect relaxing peptides. Results: The composite were characterized physicochemical properties using FTIR well peptide encapsulation efficiency, cytotoxicity, permeability through buccal intestinal cell-based models. with 0.18% (w/w) of demonstrated ca. 0.63% loading efficiency into the be safe epithelial cells up 24 h contact. assays TR146 mimic mucosa epithelium Caco-2/HT29-MTX duodenum conditions showed that incorporation guar-gum offered faster permeability, when compared free peptide-chitosan-microparticles-loaded chitosan. Conclusion: This study suggests peptide-loaded might promising carrier enhancement absorption peptide.